Identification by direct photoaffinity labeling of an altered phosphodiesterase in a mutant S49 lymphoma cell.
نویسندگان
چکیده
منابع مشابه
Identification by direct photoaffinity labeling of an altered phosphodiesterase in a mutant S49 lymphoma cell.
Extracts of a mutant S49 lymphoma cell line, termed K30a, hydrolyze cAMP and cGMP at rates much faster than do wild type S49 extracts. This elevated phosphodiesterase activity, called K-PDE, elutes as a single peak of activity on DEAE-cellulose columns (Brothers, V. M., Walker, N., and Bourne, H. R. (1982) J. Biol. Chem. 257, 9349-9355). Direct photoaffinity labeling of K30a extracts with [32P]...
متن کاملIncreased cyclic nucleotide phosphodiesterase activity in a mutant S49 lymphoma cell. Characterization and comparison with wild type enzyme activity.
We have characterized the markedly elevated cyclic AMP and cyclic GMP phosphodiesterase activities of a recently isolated S49 mouse lymphoma mutant, termed K30a, and compared both activities to enzyme activities in the parental wild type S49 cell. cAMP phosphodiesterase activity in K30a appears to be slightly larger than the major wild type CAMP-hydrolyzing enzyme, in sucrose gradient sedimenta...
متن کاملDirect photoaffinity labeling of tubulin with colchicine.
Ultraviolet irradiation of the [3H]colchicine-tubulin complex leads to direct photolabeling of tubulin with low but practicable efficiency. The bulk (70% to greater than 90%) of the labeling occurs on beta-tubulin and appears early after irradiation, whereas alpha-tubulin is labeled later. The labeling ratio of beta-tubulin to alpha-tubulin (beta/alpha ratio) is reduced by prolonged incubation,...
متن کاملThe human platelet vasopressin receptor identification by direct ultraviolet photoaffinity labeling.
Tritiated vasopressin ([3H]AVP) was directly crosslinked to its human platelet receptor by using an ultraviolet irradiation procedure. After preincubation with [3H]AVP, the hydrodynamic parameters of the hormone-receptor complexes solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate were derived from Sephacryl S-300 superfine gel filtration and from sucrose density gradie...
متن کاملAltered expression of cell cycle regulators in adult T-cell leukemia/lymphoma patients
Abstract Background: Adult T-cell leukemia/lymphoma (ATLL) is caused by human T-cell lymphotropic virus type-1 (HTLV-1). HTLV-1 oncogenes can induce malignancy through controlled gene expression of cell cycle checkpoints in the host cell. HTLV-I genes play a pivotal role in overriding cell cycle checkpoints and deregulate cellular division. In this study, we aimed to determine and comp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)44556-x